The Indica Labs Membrane FL Quantification tool measures fluorescently-labeled membranous markers on a cell-by-cell basis. The tool is color configurable and can be used to quantify any imagable fluorescent dye.
In the example above, the software measures HER2 expression in breast tissue. Each cell nucleus and cell membrane is identified and matched. Cells are then classified as negative, weak, moderate, or strong positive and cell membranes are color coded yellow, orange, and red. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score). It can also report individual expression profiles for each cell.