The Indica Labs Amplification & Deletion FISH module is one of several In–situ applications in what’s recognized as the industry’s most comprehensive ISH analytics platform. This particular module reads multi-channel fluorescent images and measures gene amplification and deletion by measuring two probes on a cell by cell basis.
In the example above the software measures HER2 amplification in breast tumor. For each cell the software reports HER2 & CEP17 copy numbers and the HER2:CEP17 ratio. It also calculates the average copy counts and ratios across all cells within the region of analysis.
The Amplification & Deletion FISH quantification tool works with most microscope images and integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats.