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HALO® Module

Axon Quantification

The Axon module counts and measures axons and myelin areas in nerve cross-sections. Since axon cross-sections are irregularly shaped, the algorithm uses image processing techniques to detect each axon’s minimum and median diameter. Once each axon has been measured the software generates important statistics including the average inner and outer diameters, average G-Ratio, average perimeter, total axon area, total myelin area, and average myelin thickness. Additionally, it produces several histograms including an axon G-Ratio histogram.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • KFBIO (KFB, KFBF)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Axon Quantification module. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Intrathecal Administration of AAV/GALC Vectors in 10- 11-Day-Old Twitcher Mice Improves Survival and Is Enhanced by Bone Marrow Transplant Karmuthil-Melethil S, Marshall MS, Heindel C, Jakubauskas B, Bongarzone ER, Gray SJ2016Journal of Neuroscience ResearchNeuroscienceAxon
Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury Stewart A, Glaser E, Mott C, Bailey W, Sullivan P, Patel S, Gensel J
2022Journal of Neurotrauma
Neuroscience
Axon
Sensory neurons display cell-type-specific vulnerability to loss of neuron-glia interactionsElbaz B, Yang L, Vardy M, Isaac S, Rader B, Kawaguchi R, Traka M, Woolf C, Renthal W, Popko B2022Cell ReportsNeuroscienceAxon
Combination of pathological, biochemical and behavioral evaluations for peripheral neurotoxicity assessment in isoniazid-treated ratsKashimura A, Nishikawa S, Ozawa Y, Hibino Y, Tateoka T, Mizukawa M, Nishina H, Sakairi T, Shiga T, Aihara N, Kamiie J2023Journal of Toxicologic PathologyToxicologyAxon

Related HALO Modules

Object Colocalization

Count one or two objects and measure the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Object Colocalization FL

Count one or two fluorescently labeled objects and measures the object area, diameter, stain intensity, and colocalization (where applicable). Microvessel and amyloid plaque quantification are most common applications.

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Microglial Activation

Quantify microglial activation based on length and thickness of microglial processes.

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Use the arrows above to view additional related modules

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