Indica Labs’ Break-Apart & Fusion FISH module is one of several In–situ applications in what’s recognized as the industry’s most comprehensive ISH analytics platform. This particular module reads multi-channel fluorescent images and quantifies break-apart assays and fusion assays in a fully automated fashion.
In the example above, the software quantitates a fusion FISH assay on lung carcinoma. The algorithm recognizes copy pairs that are overlapping or within the user-specified range to constitute a fused pair. The software reports individual cell profiles including copy counts and fusion counts. It also reports overall averages across all cells in the region of analysis.
The Break-Apart & Fusion FISH quantification tool works with most microscope images and integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats.