Indica Labs introduces the first whole slide image analysis tool for brightfield RNA ISH quantification. The patent pending software measures RNA probes on a per cell basis. The example above, shows cell nuclei (colored blue) and ISH signal (brown) that become highlighted in red/orange. The orange represents individual signals for their respective chromogens, while the red represents clustered signals that were counted as multiple signals.
The tool reports numerous metrics including cell counts, signal counts, avg signals per cell, signal sizes and optical densities, and histograms indicating the frequency of cells containing 0,1,2,3,4… signals.