The Indica Labs Cytoplasmic & Nuclear IHC quantification software can be used for a variety of IHC applications. It measures cell-by-cell IHC positivity for single or double stain applications. Each cell is individually measured for cytoplasmic and/or nuclear positivity and classified as negative, positive for stain A, positive for Stain B, or dual positive. The software also reports a variety of other statistics relating size and staining intensity metrics.
In the example shown above, the software is configured for dual-stain IHC. Pancreas tissue is analyzed and insulin+ cells are marked green, Ki67+ cells are marked red, and dual+ cells are marked cyan.