Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.
+1 (505) 492 0979
sales: info@indicalab.com
support: support@indicalab.com

FISH-IF Quantification

FISH-IF QUANTIFICATION

(Designed for RNAscope®)

 

Product Overview

Indica Labs FISH-IF quantification module measures any number of fluorescently-labeled DNA/RNA ISH probes and immunofluorescent (IF) protein biomarkers on a cell-by-cell basis. This allows the user to rapidly contextualize the corresponding protein and gene expression profile of every cell across the tissue. HALO FISH-IF analysis is designed to work with single or dual IHC-ISH assays including RNAscope™ and supports the H-score protocol for RNAscope as recommended by the manufacturer, ACD Bio.


Download the ACD Bio™ RNAscope Data Analysis Guide by clicking the links here.


An example of FISH-IF quantification is shown in the slider above.  Here, DAPI and an IF-labeled membrane biomarker (cyan) are used to identify cells of interest and the RNA FISH probe (pink) is measured in these cells.  Cells that express the membrane biomaker are highlighted in cyan.  The FISH probe spots are highlighted in pink. IF stains can be identified and quantified in any compartment – nucleus, cytoplasm and/or membrane.

The module reports numerous outputs including, but not limited to: number of IF positive cells in total and by cell compartment, Average IF intensity and average IF intensity by cell compartment, percentage IF positive total and by cell compartment, number and percentage of probe positive cells, probe copies per cell and area per cell, cell score for each probe (0, +1, +2, +3 and +4), and an H-score for each probe.  Additionally, users can define cell phenotypes based on IF and probe positivity.

The FISH-IF quantification module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

FISH-IF QUANTIFICATION

(Designed for RNAscope®)

Product Overview

Indica Labs FISH-IF quantification module measures any number of fluorescently-labeled DNA/RNA ISH probes and immunofluorescent (IF) protein biomarkers on a cell-by-cell basis. This allows the user to rapidly contextualize the corresponding protein and gene expression profile of every cell across the tissue. HALO FISH-IF analysis is designed to work with single or dual IHC-ISH assays including RNAscope™ and supports the H-score protocol for RNAscope as recommended by the manufacturer, ACD Bio.


Download the ACD Bio™ RNAscope Data Analysis Guide by clicking the links here.


An example of FISH-IF quantification is shown in the slider above.  Here, DAPI and an IF-labeled membrane biomarker (cyan) are used to identify cells of interest and the RNA FISH probe (pink) is measured in these cells.  Cells that express the membrane biomaker are highlighted in cyan.  The FISH probe spots are highlighted in pink. IF stains can be identified and quantified in any compartment – nucleus, cytoplasm and/or membrane.

The module reports numerous outputs including, but not limited to: number of IF positive cells in total and by cell compartment, Average IF intensity and average IF intensity by cell compartment, percentage IF positive total and by cell compartment, number and percentage of probe positive cells, probe copies per cell and area per cell, cell score for each probe (0, +1, +2, +3 and +4), and an H-score for each probe.  Additionally, users can define cell phenotypes based on IF and probe positivity.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Roche Ventana (BIF)
Philips (Requires Philips IMS)

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.