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HALO® Module

Islet Quantification FL

Indica Labs’ Islet Quantification FL module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and nuclear staining.

The module can also analyze two cytoplasmic markers, for example insulin and glucagon. In cases where tissues are probed for two islet markers such as glucagon and insulin, the islet boundary can be determined based on positivity for both markers.

File formats supported by the HALO image analysis platform:

  • Non-proprietary (JPG, TIF, OME.TIFF)
  • Nikon (ND2)
  • 3D Histech (MRXS)
  • Akoya (QPTIFF, component TIFF)
  • Olympus / Evident (VSI)
  • Hamamatsu (NDPI, NDPIS)
  • Aperio (SVS, AFI)
  • Zeiss (CZI)
  • Leica (SCN, LIF)
  • Ventana (BIF)
  • Philips (iSyntax, i2Syntax)
  • KFBIO (KFB, KFBF)
  • DICOM (DCM*)
    *whole-slide images

Publication Spotlight

The table below includes publications that cite the Islet IHC or FL modules. 

Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Single-Cell RNAseq Reveals That Pancreatic β-Cells From Very Old Male Mice Have a Young Gene SignatureXin Y, Kim J, Okamoto H, Ni M, Wei Y, Adler C, Murphy AJ, Yancopoulos GD, Lin C, Gromada J2016EndocrinologyMetabolismIslet

Related HALO Modules

Highplex FL

Quantify expression of an unlimited number of biomarkers in any cellular compartment - membrane, nucleus or cytoplasm.

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Tissue Classifier Add-On

Separate multiple tissue classes across a tissue using a learn-by-example approach. Can be used in conjunction with all other modules (fluorescent and brightfield) to select specific tissue classes for further analysis.

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Vacuole Quantification

Quantifies vacuole area, diameter, perimeter, and number of vacuoles per region of interest. Measures steatosis in kidney, lipid droplets in adipose tissue, and alveolar area in lung tissue.

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