Indica Labs’ Islet FL Quantification tool counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and nuclear staining.
In the example above, pancreas sections are probed with DAPI (blue), anti-insulin antibodies detected with a red fluorophore and anti-Ki67 antibodies detected with a green fluorophore. The software identifies the outer islet boundaries based on insulin positivity. Cells within the islet boundary are then scored for insulin and Ki67 positivity. In the markup image, insulin-positive beta cells are identified with red cytoplasmic markup, Ki-67 positive cells are identified with green nuclear markup, dual-positive cells have both red and green markup, and cells with no staining have blue nuclear markup. The software reports number of islets, islet area, number of insulin, Ki-67, dual-positive cells, as well as the area of positive stains across the area analyzed and within each islet identified.
The software can also analyze two cytoplasmic markers, for example insulin and glucagon. In cases where tissues are probed for two islet markers such as glucagon and insulin, the islet boundary can be determined based on positivity for both markers