The Indica Labs Object Colocalization FL tool provides flexible, fully automated, object based analysis of whole-slide fluorescence images. This tool reads multi-channel images, locating and counting one or two types of objects and measuring co-localization when applicable. Each object type is detected based on size and shape. The software reports the number of each object type and the percentage of overlapping (co-localizing) area with the second object type. Optionally, the tool can output the complete table of object data including individual object sizes, overlapping percentages, and intensity measurements for each dye.
In the example above the software identifies both vessels and amyloid plaques in mouse brain. Vessels are marked red, plaques are marked green, and co-localizing regions are marked yellow.
The Object Colocalization FL tool can be used with most fluorescent microscopes and integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats.