Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane. With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya Vectra® 3 and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Akoya (CODEX® Platform), Ultivue (UltiMapper and InSituPlex® Technology), Fluidigm (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).
In the example shown here, the fluorescent image displays five colors – a membrane bound tumor marker in red, a cytoplasmic marker in cyan, two distinct nuclear markers in green and fuschia and blue DAPI counterstain. In the markup image, the nucleus, cytoplasm and membranes are colored based on the cell positivity for each marker. Cells negative for all markers are black, cells positive for multiple markers are white, and cells positive for individual makers are colored according to that marker color. When used in conjunction with our Spatial Analysis module, the spatial relationships of these cell populations may be further interrogated.
The Highplex FL module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats. If you don’t see your format listed here, it doesn’t necessarily mean that we don’t support it. Drop an email to firstname.lastname@example.org to check with us directly.