The Indica Labs Membrane IHC Quantification tool measures membranous immunohistochemical markers on a cell-by-cell basis. This tool is color configurable, so it can be used with non-traditional chromogens as well as with DAB.
In the example above, the software measures HER2 expression in breast tissue. Each cell nucleus and cell membrane is identified and matched. Cells are then classified as negative, weak, moderate, or strong positive and cell membranes are color coded yellow, orange, and red. The software reports overall scores and positivity percentages for the entire analysis region (including H-Score). It can also report individual expression profiles for each cell.