Indica Labs’ Multiplex DNA FISH module is one of several In–situ applications in what’s recognized as the industry’s most comprehensive ISH analytics platform. This particular module reads multi-channel fluorescent images and measures up to four DNA FISH probes on a cell-by-cell basis allowing the user to generate thousands of single-cell DNA expression profiles in seconds. In the example above the software measures a four-plex CymogenDx assay. Each cell is identified by nuclear detection on the DAPI channel, and the four probes (red, green, aqua, and orange) are measured within the cell boundaries. The software reports the signal count for each probe across the entire region of analysis as well as the individual expression profiles for each cell.
The Multiplex DNA FISH quantification tool works with most microscope and whole-slide-scanner images and integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats.