Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Multiplex IHC

MULTIPLEX IHC

 

Product Overview

Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications.  This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity.  The software also reports a variety of other statistics relating size and staining intensity metrics.

In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain.  Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue).  In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue.  Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink.  Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.

The Multiplex IHC module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

MULTIPLEX IHC

Indica Labs’ Multiplex IHC module separates up to five stains simultaneously within any cellular compartment (nucleus, cytoplasm or membrane) and can be used for a variety of IHC applications. This module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue. Each cell is individually measured for cytoplasmic, nuclear, and/or membrane positivity. The software also reports a variety of other statistics relating size and staining intensity metrics.

In the example above, a tissue core is stained with a brown (DAB) nuclear marker, blue membrane marker, pink/red cytoplasmic marker, and nuclear hematoxylin counterstain. Cells are segmented using a combination of the two nuclear stains (hematoxylin and DAB) and one membrane stain (blue). In the corresponding markup image, positive nuclei are colored brown and negative nuclei colored blue. Similarly, cells identified as positive for the membrane marker are colored blue and cells identified as positive for cytoplasmic marker are pink. Advanced staining courtesy of the Trev and Joyce Deeley Research Centre, BC Cancer Agency.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.