Indica Labs introduces the most advanced image analysis tool for muscle fiber detection and quantification on whole slide images. The patent pending software detects muscle fibers then reports the total count of muscle fibers and calculates the perimeter, area, and diameter of each fiber.
Since muscle fiber cross-sections are irregularly shaped, the algorithm uses patent pending image processing techniques to detect each fiber’s minimum diameter (green lines) and maximum diameter (blue lines). Finally software reports the average minimum and maximum diameters, the average perimeter length, and the average fiber area for all fibers in the entire section.
As in the example shown above, the software is capable of detecting unstained muscle fibers where fiber basement membranes are stained, in this case with Laminin. It can also be configured to detect nuclei and identify fibers with centrally located nuclei. A notable increase or decrease in fibers with centrally located nuclei can be indicative of certain myopathic findings.