Indica Labs’ Serial Section Analysis module can be used to create a classifier based off of a reference slide (e.g., H&E), and apply that slide to serial sections stained for different IHC Markers (e.g., HER2, ER, PR).
In the example above, a tissue biopsy section is stained with hematoxylin and eosin. A classifier has been created segmenting tumor from stroma (Tumor in red, stroma in green). The software takes the Classifier created from the H&E image and allows you to apply the classified image to IHC-stained serial sections (e.g., ER, HER2) as regions of interest. The tumor regions, based off of the H&E classifier, are analyzed on the ER and HER2 slides using the Cytonuclear Module and Membrane Module, respectively.