Indica Labs introduces the most advanced image analysis tool for SISH, CISH, and dual ISH quantification on whole slide images. The patent pending software measures gene amplification by counting probe signals within each cell’s nucleus.
The example above, shows cell nuclei (colored blue) and Dual CISH signals (red and black) that become highlighted in red/orange and cyan/green, respectively. The orange and cyan represent individual signals for their respective chromogens, while the green and red represent clustered signals that were counted as multiple signals.
Finally, the software reports the total number of cells, total number of spots, the average number of spots per cell, as well as a histogram displaying the frequency of cells containing 0,1,2,3,4… signals. In the case of dual CISH, the software reports Her2 to Chr17 ratios on a per cell basis
The intended use for the SISH & CISH quantification software is for research purposes only. The tool integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and file formats.