Indica Labs’ Spatial Analysis module is a suite of tools for analyzing proximity and relative spatial distribution of objects, cells, and/or features across single tissues or serial sections. This module is of particular importance for those conducting immuno-oncology and angiogenesis research. The module runs within our HALO platform and can be used in conjunction with any of our cell-based analysis modules for brightfield or fluorescence.
Nearest Neighbor Analysis
Determine the average distance and number of unique neighbors between any two cell or object populations.
The example below, shows pan cytokeratin positive cells (blue) and CD4 positive cells (red). The grey lines connect each CD4 positive cell to the nearest pan cytokeratin positive cell.
Calculate the number of objects or cells within a certain distance of another object or cell.
In the example below, this distance is set to 30 microns. CD8 positive cells within 30 microns of a pan cytokeratin positive cell (blue) are labeled green and CD8 positive cells greater than 30 microns from a pan cytokeratin positive cell are labeled purple. A corresponding proximity histogram is automatically generated.
Determine the number of objects or cells within a certain distance of an annotation region.
Shown below is the automatically generated histogram displaying the density. In this example the distance is set to 500 microns around the drawn tumor boundary.