Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Highplex FL

HIGHPLEX FL

 

Product Overview

Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane.  With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya PhenoImager (Phenoptics), PhenoCycler (CODEX®), Vectra® 3, and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Lunaphore (OME.TIFF images from the COMET™ Platform), Ultivue (FixVUE™, FlexVUE™, U-Vue® Panels and InSituPlex® Technology), Standard BioTools (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).

In the example shown here, the fluorescent image displays five colors – a membrane bound tumor marker in red, a cytoplasmic marker in cyan, two distinct nuclear markers in green and fuschia and blue DAPI counterstain.  In the markup image, the nucleus, cytoplasm and membranes are colored based on the cell positivity for each marker. Cells negative for all markers are black, cells positive for multiple markers are white, and cells positive for individual makers are colored according to that marker color. When used in conjunction with our Spatial Analysis module, the spatial relationships of these cell populations may be further interrogated.

The Highplex FL module integrates seamlessly into the HALO® platform which is compatible with a number of third party systems and  file formats. If you don’t see your format listed here, it doesn’t necessarily mean that we don’t support it. Drop an email to info@indicalab.com to check with us directly.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

HIGHPLEX FL

Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane.  With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya PhenoImager (Phenoptics), PhenoCycler (CODEX®), Vectra® 3, and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Lunaphore (OME.TIFF images from the COMET™ Platform), Ultivue (FixVUE™, FlexVUE™, U-Vue® Panels and InSituPlex® Technology), Standard BioTools (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).

 

In the example shown here, the fluorescent image displays five colors – a membrane bound tumor marker in red, a cytoplasmic marker in cyan, two distinct nuclear markers in green and fuschia and blue DAPI counterstain.  In the markup image, the nucleus, cytoplasm and membranes are colored based on the cell positivity for each marker. Cells negative for all markers are black, cells positive for multiple markers are white, and cells positive for individual makers are colored according to that marker color. When used in conjunction with our Spatial Analysis module, the spatial relationships of these cell populations may be further interrogated.

Posters
eBooks
Publications

Here are a few publications that cite use of the Highplex FL module.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Integrative molecular characterization of sarcomatoid and rhabdoid renal cell carcinomaBakouny Z, Braun DA, Shukla SA, Pan W, Gao X, Hou Y, Flaifel A, Tang S, Bosma-Moody A, He MX, Vokes N, Nyman J, Xie W, Nassar AH, Alaiwi SA, Flippot R, Bouchard G, Steinharter JA, Nuzzo PV, Ficial M, Sant’Angelo M, Forman J, Berchuck JE, Dudani S, Bi K, Park J, Camp S, Sticco-Ivins M, Hirsch L, Baca SC, Wind-Rotolo M, Ross-Macdonald P, Sun M, Mary Lee G-SM, Chang SL, Wei XX, McGregor BA, Harshman LC, Genovese G, Ellis L, Pomerantz M, Hirsch MS, Freedman ML, Atkins MB, Wu CJ, Ho TH, Linehan WM, McDermott DF, Heng DYC, Viswanathan SR, Signoretti S, Van Allen EM, Choueiri TK2021Nature CommunicationsOncologyHighplex FL
Human ileal organoid model recapitulates clinical incidence of diarrhea associated with small molecule drugsBelair DG, Visconti RJ, Hong M, Marella M, Peters MF, Scott CW, Kolaja KL2020Toxicology in VitroToxicologyHighplex FL
IgA transcytosis and antigen recognition govern ovarian cancer immunityBiswas S, Mandal G, Payne KK, Anadon CM, Gatenbee CD, Chaurio RA, Costich TL, Moran C, Harro CM, Rigolizzo KE, Mine JA, Trillo-Tinoco J, Sasamoto N, Terry KL, Marchion D, Buras A, Wenham RM, Yu X, Townsend MK, Tworoger SS, Rodriguez CR, Anderson AR, Conejo-Garcia JR2021NatureImmuno-oncologyHighplex FL
Spatial Clustering of CD68+ Tumor Associated Macrophages with Tumor Cells is Associated with Worse Overall Survival in Metastatic Clear Cell Renal Cell CarcinomaChakiryan NH, Kimmel GJ, Kim Y, Hajiran A, Aydin AM, Zemp L, Katende E, Nguyen J, Lopez-Blanco N, Chahoud J, Spiess PE, Fournier M, Dhillon J, Wang L, Moran-Segura C, El-Kenawi A, Mulé J, Altrock PM, Manley BJ2021bioRxivImmuno-oncologyHighplex FL
Multiomic analysis and immunoprofiling reveal distinct subtypes of human angiosarcomaChan JY, Lim JQ, Yeong J, Ravi V, Guan P, Boot A, Tay TKY, Selvarajan S, Nasir NDM, Loh JH, Ong CK, Huang D, Tan J, Li Z, Ng CC-Y, Tan TT, Masuzawa M, Sung KW-K, Farid M, Quek RHH, Tan NC, Teo MCC, Rozen SG, Tan P, Fotreal A, The BT, Soo KC2020The Journal of Clinical InvestigationImmuno-oncologyHighplex FL
Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma Cloughesy TF, Mochizuki AY, Orpilla JR, Hugo W, Lee AH, Davidson TB, Wang AC, Ellingson BM, Rytlewski JA, Sanders CM, Kawaguchi ES, Du L, Li G, Yong WH, Gaffey SC, Cohen AL, Mellinghoff IK, Lee EQ, Reardon DA, O’Brien BJ, Butowski NA, Nghiemphu PL, Clarke JL, Arrillaga-Romany IC, Colman H, Kaley TJ, de Groot JF, Liau LM, Wen PY, Prins RM2019Nature MedicineOncology, Immuno-oncologySpatial Analysis, Highplex FL
Characterization of Macrophages and Osteoclasts in the Osteosarcoma Tumor Microenvironment at Diagnosis: New Perspective for Osteosarcoma Treatment?Gomez-Brouchet A, Gilhodes J, Van Acker N, Brion R, Bouvier C, Assemat P, Gaspar N, Aubert S, Guinebretiere J-M, Marie B, Larousserie F, Entz-Werlé N, de Pinieux G, Mascard E, Gouin F, Brousset P, Tabone M-D, Jimenez M, Le Deley M-C, Blay J-Y,
Brugieres L , Piperno-Neumann S, Rédini F
2021CancersImmuno-oncologyHighplex FL
Tumoral PD-1hiCD8+ 1 T cells are partially exhausted and 2 predict favorable outcome in triple-negative breast cancer Guo L, Cao C, Goswami S, Huang X, Ma L, Guo Y, Yang B, Li T, Chi Y, Zhang X, Wu J2020Clinical ScienceImmuno-oncologyHighplex FL
Reduction of Global H3K27me3 Enhances HER2/ErbB2 Targeted TherapyHirukawa A, Singh S, Wang J, Rennhack JP, Swiatnicki M, Sanguin-Gendreau V, Zuo D, Daldoul K, Lavoie C, Park M, Andrechek ER, Westbrook TF, Harris LN, Varadan V, Smith HW, Muller WJ2019Cell ReportsOncologyHighplex FL
Macrophage-Derived CXCL9 and CXCL10 Are Required
for Antitumor Immune Responses Following Immune
Checkpoint Blockade
House IG, Savas P, Lai J, Chen AXY, Oliver AJ, Teo ZL, Todd KL, Henderson MA, Giuffrida L, Petley EV, Sek K, Mardiana S, Gide TN, Quek C, Scolyer RA, Long GV, Wilmott JS, Loi S, Darcy PK, Beavis PA2019Clinical Cancer ResearchImmuno-oncologyHighplex FL
A single dose of neoadjuvant PD-1 blockade predicts clinical outcomes in resectable melanomaHuang AC, Orlowski RJ, Xu X, Mick R, George SM, Yan PK, Manne S, Kraya AA, Wubbenhorst B, Dorfman L, D’Andrea K, Wenz BM, Liu S, Chilukuri L, Kozlov A, Carberry M, Giles L, Kier MW, Quagliarello F, McGettigan S, Kreider K, Annamalai L, Zhao Q, Mogg R, Xu W, Blumenschein WM, Yearley JH, Linette GP, Amaravadi RK, Schuchter LM, Herati RS, Bengsch B, Nathanson KL, Farwell MD, Karakousis GC, Wherry EJ, Mitchell TC2019Nature MedicineOncology, Immuno-oncologyHighplex FL
CXCR4+ cells are increased in lung tissue of patients with idiopathic pulmonary fibrosisJaffar J, Griffiths K, Oveissi S, Duan M, Foley M, Glaspole I, Symons K, Organ L, Westfall G2020Respiratory ResearchFibrosisHighplex FL
Neutrophil content predicts lymphocyte depletion and anti-PD1 treatment failure in NSCLCKargl J, Zhu X, Zhang H, Yang GHY, Friesen TJ, Shipley M, Maeda DY, Zebala JA, McKay-Fleisch J, Meredith G, Mashadi-Hossein A, Baik C, Pierce RH, Redman MW, Thompson JC, Albelda SM, Bolouri H, Houghton AM2019JCI InsightHighplex FL
CD30+OX40+ Treg is associated with improved overall survival in colorectal cancerLam JH, Hong M, Koo S-L, Chua CWL, Lim KL, Wee F, Wan WK, Leow WQ, Yeo JG, Tan IBH, Yeong J, Lim TKH, Lim TS2021Cancer Immunology, ImmunotherapyImmuno-oncologyHighplex FL
CXCR3 enables recruitment and site-specific bystander activation of memory CD8+ T cellsMaurice NJ, McElrath MJ, Andersen-Nissen E, Frahm N, Prlic M2019Nature CommunicationsImmunologyHighplex FL
MYB-NFIB gene fusions identified in archival adenoid cystic carcinoma tissue employing NanoString analysis: an exploratory study McIntyre JB, Ko JJ, Siever J, Chan AMY, Simpson RHW, Hao D, Lau HY2019Diagnostic PathologyOncologyClassifier, Highplex FL
Sustained Helicobacter pylori infection accelerates gastric dysplasia in a mouse modelO’Brien VP, Koehne AL, Dubrulle J, Rodriguez AE, Leverich CK, Kong VP, Campbell JS, Pierce RH, Goldenring JR, Choi E, Salama NR2021Life Science AllianceImmuno-oncologyHighplex FL
Locally confined IFNɣ production by CD4+ T cells provides niches for murine cytomegalovirus
4 replication in the salivary gland
Oderbolz J, Zangger N, Zimmerman L, Sandu I, Starruß J, Graw F, Oxenius A2021bioRxivClassifier, Spatial Analysis, Highplex FL
Pulmonary Mycobacterium tuberculosis control associates with CXCR3- and CCR6-expressing antigen-specific Th1 and Th17 cell recruitmentShanmugasundaram U, Bucsan AN, Ganatra SR, Ibegbu C, Quezada M, Blair RV, Alvarez X, Velu V, Kaushal D, Rengarajan J2020JCI InsightInfectious DiseaseHighplex FL, HALO AI
T-cell Localization, Activation, and Clonal Expansion in Human Pancreatic Ductal Adenocarcinoma Stromnes IM, Hulbert A, Pierce RH, Greenberg PD, Hingorani SR2017Cancer Immunology ResearchOncology, Immuno-oncologyHighplex FL
Rova-T enhances the anti-tumor activity of anti-PD1 in a murine model of small cell lung cancer with endogenous Dll3 expressionVitorino P, Chuang C-H, Iannello Z, Zhao X, Anderson W, Ferrando R, Zhang Z, Madhavan S, Karsunky H, Saunders LR2020Translational OncologyImmuno-oncologyHighplex FL
Validation and characterisation of prognostically significant PD-L1+ immune cells in HPV+ oropharyngeal squamous cell carcinomaYoung RJ, Bressel M, Porceddu S, Cernelc J, Savas P, Liu H, Urban D, Thai AA, Cooper C, Fua T, Neeson P, Rischin D, Solomon B2019Oral OncologyOncology, Infectious DiseaseClassifier, Highplex FL
Safety, immunogenicity, and clinical efficacy of durvalumab in combination with folate receptor alpha vaccine TPIV200 in patients with advanced ovarian cancer: a phase II trialZamarin D, Walderich S, Holland A, Zhou Q, Iasonos AE, Torrisi JM, Merghoub T, Chesebrough LF, Mcdonnell AS, Gallagher JM, Li Y, Hollmann TJ, Grisham RN, Erskine CL, Block MS, Knutson KL, O'Cearbhaill RE, Aghajanian C, Konner JA2020Journal for ImmunoTherapy of CancerImmuno-oncologyHighplex FL
Trajectory and Functional Analysis of PD-1high CD4+CD8+ T Cells in Hepatocellular Carcinoma by Single-Cell Cytometry and Transcriptome SequencingZheng B, Wang D, Qiu X, Luo G, Wu T, Yang S, Li Z, Zhu Y, Wang S, Wu R, Sui C, Gu Z, Shen S, Jeong S, Wu Z, Gu J, Wang H, Chen L2020Advanced ScienceImmuno-oncologyHighplex FL
Genetic alterations and expression characteristics of ARID1A impact tumor immune contexture and survival in early-onset gastric cancerZou J, Qin W, Wang L, Wang Y, Shen J, Xiong W, Yu S, Song S, Ajani JA, Lin S-Y, Mills GB, Yuan X, Chen J, Peng G2020American Journal of Cancer ResearchImmuno-oncologyMicroglia, Highplex FL
CD38 in Advanced Prostate CancersGuo C, Crespo M, Gurel B, Dolling D, Rekowski J, Sharp A, Petremolo A, Sumanasuriya S, Rodrigues DN, Ferreira A, Pereira R, Figueiredo I, Mehra N, Lambros MBK, Neeb A, Gil V, Seed G, Terstappen L, Alimonti A, Drake CG, Yuan W, de Bono JS2021European UrologyOncologyClassifier, Highplex FL
Influence of tumor-infiltrating immune cells on local control rate, distant metastasis, and survival in patients with soft tissue sarcomaSmolle MA, Herbsthofer L, Goda M, Granegger B, Brcic I, Bergovec M, Scheipl S, Prietl B, El-Heliebi A, Pichler M, Gerger A, Posch F, Tomberger M, López-García P, Feichtinger J, Baumgartner C, Leithner A, Liegl-Atzwanger B, Szkandera J2021OncoImmunologyImmuno-oncologyHighplex FL
Activated Regulatory T-Cells, Dysfunctional and SenescentT-Cells Hinder the Immunity in Pancreatic CancerSivakumar S, Abu-Shah E, Ahern DJ, Arbe-Barnes EH, Jainarayanan AK, Mangal N, Reddy S, Rendek A, Easton A, Kurz E, Silva M, Soonawalla Z, Heij LR, Bashford-Rogers R, Middleton MR, DUstin ML2021cancersClassifier, Multiplex IHC, Spatial Analysis, Highplex FL
The immune suppressive microenvironment affects efficacy of radio-immunotherapy in brain metastasisNiesel K, Schulz M, Anthes J, Alekseeva T, Macas J, Salamero-Boix A, Mockl A, Oberwahrenbrock T, Lolies M, Stein S, Plate KH, Reiss Y, Rodel F, Sevenich L2021EMBO Molecular MedicineImmuno-oncologySpatial Analysis, Highplex FL
Prognostic role of proliferating CD8+ cytotoxic Tcells in human cancersBlessin NC, Li W, Mandelkow T, Jansen HL, Yang C, Raedler JB, Simon R, Büscheck F, Dum D, Luebke AM, Hinsch A, Möller K, Menz A, Bernreuther C, Lebok P, Clauditz T, Sauter G, Marx A, Uhlig R, Wilczak W, Minner S, Krech T, Fraune C, Höflmayer D, Burandt E, Steurer S2021Cellular OncologyImmuno-oncologyHighplex FL, TMA
Engineered red blood cells as an off-the-shelf allogeneic anti-tumor therapeuticZhang X, Lou M, Dastagir SR, Nixon M, Khamhoung A, Schmidt A, Lee A, Subbiah N, McLaughlin DC, Moore CL, Gribble M, Bayhi N, Amin V, Pepi R, Pawar S, Lyford TJ, Soman V, Mellen J, Carpenter CL, Turka LA, Wickham Tj, Chen TF2021Nature CommunicationsOncologyObject Colocalization, Highplex FL
Heterogeneity in tertiary lymphoid structure B-cells correlates with patient survival in metastatic melanomLynch K, Young S, Menaveau M, et al.2021Journal for Immunotherapy of CancerImmuno-oncologyHighplex FL
Tumor necroptosis is correlated with a favorable immune cell signature and programmed death-ligand 1 expression in cholangiocarcinomaLomphithak T, Akara-amornthum P, Murakami K, et al.2021Scientific ReportsImmuno-oncologyHighplex FL
Mismatch repair-deficient prostate cancer with parenchymal brain metastases treated with immune checkpoint blockadeSena L, Salles D, Engle E, Zhu Q, Tukachinsky H, Lotan T, Antonarakis E2021Molecular Case StudiesImmuno-oncologyHighplex FL
Dissecting spatial heterogeneity and the immuneevasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinomaSun Y, Wu L, Liu S, Jiang M, Hu B, Zhou K, Guo W, Xu Y, Zhong Y, Zhou X, Zhang Z, Liu G, Liu S, Shi Y, Ji Y, Du M, Li N, Li G, Zhao Z, Huang X, Xu L, Yu Q, Peng D, Qiu S, Sun H, Dean M, Wang X, Chung W, Dennison A, Zhou J, Hou Y, Fan J, Yang X2021Nature CommunicationsOncology, Immuno-oncologyHighplex FL
Anti-tumor effects of RTX-240: an engineered red blood cell expressing 4-1BB ligand and interleukin-15McArdel S, Dugast A, Hoover M, Bollampalli A, Hong E, Castano Z Leonard S, Pawar S, Mellen J, Muriuki K, McLaughlin D, Bayhi N, Carpenter C, Turka L, Wickham T, Elloul S2021Cancer Immunology, ImmunotherapyImmuno-oncologyHighplex FL
Distinct immune signatures in peripheral blood predict chemosensitivity in intrahepatic cholangiocarcinoma patientsWu T, Yang Y, Zheng B, Shi X, Li W, Ma W, Wang S, Li Z, Zhu Y, Wu J, Wang K, Zhao Y, Wu R, Sui C, Shen S, Wu X, Chen L, Yuan Z, Wang H2021EngineeringOncology, ImmunologyHighplex FL
The Unfolded Protein Response Mediator PERK Governs Myeloid Cell-Driven Immunosuppression in Tumors through Inhibition of STING SignalingMohamed E, Sierra RA, Trillo-Tinoco J, Cao Y, Innamarato P, Payne KK, Pulido M, Mandula J, Zhang S, Thevenot P, Biswas S, Abdalla SK, Costich TL, Hanggi K, Anadon CM, Flores ER, Haura EB, Mehrotra, Pilon-Thomas S, Ruffel B, Munn DH, Cubillos-Ruiz JR, Conejo-Garcia JR, Rodriguez PC2020ImmunityImmuno-oncologyHighplex FL
The InSituPlex® Staining Method for Multiplexed Immunofluorescence Cell Phenotyping and Spatial Profiling of Tumor FFPE SamplesManesse M, Patel KK, Bobrow M, Downing SR2020Biomarkers for Immunotherapy of CancerOncology, Immuno-oncologyHighplex FL
Highly efficient genome editing in primary human bronchial epithelial cells differentiated at air-liquid interfaceRapiteanu R, Karagyozova T, Zimmerman N, Singh K, Wayne G, Martufi M, Belyaev NN, Hessel EM, Michalovich D, Macarron R, Rowan WC, Cairns WJ, Roger J, Betts J, Beinke S, Maratou K2020European Respiratory JournalOtherHighplex FL
A Dedicated Evolutionarily Conserved Molecular Network Licenses Differentiated Cells to Return to the Cell CycleMiao Z-F, Lewis MA, Cho CJ, Adkins-Threats M, Park D, Brown JW, Sun J-X, Burclaff JR, Kennedy S, Lu J, Mahar M, Vietor I, Huber LA, Davidson NO, Cavalli V, Rubin DC, Wang Z-N, Mills JC2020Developmental CellOncologyHighplex FL
Chemoradiotherapy efficacy is predicted by intra-tumour CD8+/FoxP3+ double positive T cell density in locally advanced N2 non–small-cell lung carcinomaBoulle G, Velut Y, Mansuet-Lupo A, Gibault L, Blons H, Fournel L, Boni A, Cremer I, Wislez M, Duchatelle V, Tredaniel J, Hammond SA, Herbst R, Alifano M, Giraud P, Damotte D2020European Journal of CancerOncologyHighplex FL
RNA isoform screens uncover the essentiality and tumor-suppressor activity of ultraconserved poison exonsThomas JD, Polaski JT, Feng Q, De Neef EJ, Hoppe ER, McSharry MV, Pangallo J, Gabel AM, Belleville AE, Watson J, Nkinsi NT, Berger AH, Bradley RK2020Nature GeneticsOtherHighplex FL
High density of cytotoxic T-lymphocytes is linked to tumoral PD-L1 expression regardless of the mismatch repair status in colorectal cancerMöller K, Blessin N, Höflmayer D, et al.2021Acta OncologicaImmuno-oncologyHighplex FL
Selective loss of resident macrophage-derived insulin-like growth factor-1 abolishes adaptive cardiac growth to stressZaman R, Hamidzada H, Kantores C, Wong A, Dick S, Wang Y, Momen A, Aronoff L, Lin J, Razani B, Mital S, Billia F, Lavine K, Nejat S, Epelman S2021ImmunityImmunologyHighplex FL
NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancerGuo H, Golczer G, Wittner B, Langenbucher A, Zachariah M, Dubash T, Hong X, Comaills V, Burr R, Ebright R, Horwitz E, Vuille J, Hajizadeh S, Wiley D, Reeves B, Zhang J, Niederhoffer K, Lu C, Wesley B, Ho U, Nieman L, Toner M, Vasudevan S, Zou L, Mostoslavsky R, Maheswaran S, Lawrence M, Haber D2021Molecular CellOncologyHighplex FL
Inhibitory effects and mechanisms of the anti-covid-19 traditional Chinese prescription, Keguan-1, on acute lung injuryBai, Z, Li P, Wen J, Han Y, Cui Y, Zhou Y, Shi Z, Chen S, Li Q, Zhao X, Wang Z, Li R, Guo Y, Zhan X, Xu G, Ding K, Wang J, Xiao X2021Journal of EthnopharmacologyInfectious DiseaseHighplex FL
Spatial Clustering of CD68+ Tumor Associated Macrophages with Tumor Cells is Associated
with Worse Overall Survival in Metastatic Clear Cell Renal Cell Carcinoma
Chakiryan NH, Kimmel GJ, Kim Y, Hajiran A, Aydin AM, Zemp L, Katende E, Nguyen J, Lopez-Blanco N, Chahoud J, Spiess PE, Fournier M, Dhillon J, Wang L, Moran-Segura C, El-Kenawi A, Mulé J, Altrock PM, Manley BJ2021PLOS ONEImmuno-oncologyHighplex FL
A locus coeruleus to dentate gyrus noradrenergic circuit modulates aversive contextual processingSeo D, Zhang E, Piantadosi S, et al2021Neuron NeuroscienceArea Quantification, Highplex FL
Preoperative ipilimumab plus nivolumab in locoregionally advanced urothelial cancer: the NABUCCO trialvan Dijk N, Gil-Jimenez A, Silina K, Hendricksen K, Smit LA, de Feijter JM, van Montfoort ML, van Rooijen C, Peters D, Broeks A, van der Poel HG, Bruining A, Lubeck Y, Sikorska K, Boellaard TN, Kvistborg P, Vis DJ, Hooijberg E, Schumacher TN, van den Broek M, Wessels LFA, Blank CU, van Rhijn BW, van der Heijden MS2020Nature MedicineImmuno-oncologyClassifier, Highplex FL
Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent antitumor immunity in melanomaJacquelot N, Seillet C, Wang M et al.2021Nature ImmunologyImmuno-oncologyClassifier, Highplex FL
Association between CD8 and PD?L1 expression and outcomes after radical prostatectomy for localized prostate cancerVicier C, Ravi P, Kwak L, Werner L, Huang Y, Evan C, Loda M, Hamid AA, Sweeney CJ2020The ProstateImmuno-oncologyClassifier, Highplex FL
AG-205 Upregulates Enzymes Involved in Cholesterol Biosynthesis and Steroidogenesis in Human Endometrial Cells Independently of PGRMC1 and Related MAPR ProteinsThieffry C, Van Wynendaele M, Aynaci A, Maja M, Dupuis C, Loriot A, Marbaix E, Henriet P2021BiomoleculesOtherCytonuclear, Highplex FL
An endogenous opioid circuit determines state-dependent appetitive behaviorCastro D, Oswell C, Zhang E, Pedersen C, Piantadosi S, Rossi M, Hunker A, Guglin A, Moron J, Zweifel L, Stuber G, Bruchas M2021bioRxivNeuroscienceCytonuclear, Highplex FL
Maternal platelets pass interstices of trophoblast columns and are not activated by HLA-G in early human pregnancyGuettler J, Forstner D, Cvirn G, Maninger S, Brugger B Nonn O, Kupper N, Pritz E, Wernitznig S, Dohr G, Hutter H, Juch H, Isermann B, Kohli S, Gauster M2021Journal of Reproductive ImmunologyOtherCytonuclear, Highplex FL
Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancersNewman J, Gavard A, Lieb S, Ravichandran M, Hauer K, Werni P, Geist L, Bottcher J, Engen J, Rumpel K, Samwer M, Petronczki, Gileadi O2021Life Science AllianceOncologyCytonuclear, Highplex FL
MNK inhibition sensitizes KRAS-mutant colorectal cancer to mTORC1 inhibition by reducing eIF4E phosphorylation and c-MYC expressionKnight J, Alexandrou C, Skalka G, Vlahov N, Pennel K, Officer L, Teodosio A, Kanellos G, Gay D, May-Wilson S, Smith E, Najumudeen A, Gilroy K, Ridgway R, Flanagan D, Smith R, McDonald L, MacKay C, Cheasty A, McArthur K, Stanway E, Leach J, Jackstadt R, Waldron J, Campbell A, Vlachogiannis G, Valeri N, Haigis K, Sonenberg N, Proud C, Jones N, Swarbrick M, McKinnon H, Faller W, LeQuense J, Edwards J, Willis A, Bushell M, Sansom O2021Cancer DiscoveryOncologyCytonuclear, Highplex FL
CD98-induced CD147 signaling stabilizes the Foxp3 protein to maintain tissue homeostasisGeng J, Chen R, Yang F, Lin P, Zhu Y, Fu X, Wang K, Feng Z, Wu J, Zhang H, Li Q, Chen Z, Zhu P2021Cellular and Molecular ImmunologyImmuno-oncologyCytonuclear, Highplex FL
EHF is essential for epidermal and colonic epithelial homeostasis and suppresses Apc-initiated colonic tumorigenesisReehorst C, Nightingale R, Luk I, Jenkins L, Koentgen F, Williams D, Darido C, Tan F, Anderton H, Chopin M, Schoffer K, Eissmann M, Buchert M, Mouradov D, Sieber O, Ernst M, Dhillon A, Maridason J2021DevelopmentOncologyCytonuclear, Highplex FL
Antiretroviral therapy timing impacts latent tuberculosis infection reactivation in a tuberculosis/simian immunodeficiency virus coinfection modelSharan R, Ganatra S, Bucsan A, Cole J, Singh D, Alvarez X, Gough M, Alvarez C, Blakley A, Ferdin J, Thippeshappa R, Singh B, Escobedo R, Shivanna V, Dick E, Hall-Ursone S, Khader S, Mehra S, Rengarajan J, Kaushal D2021The Journal of Clinical InvestigationInfectious DiseaseCytonuclear, Highplex FL
Discoidin Domain Receptor 1 (DDR1) is Necessary for Tissue Homeostasis in Pancreatic Injury and Pathogenesis of Pancreatic Ductal AdenocarcinomaRuggeri JM, Franco-Barraza J, Sohail A, Zhang Y, Long D, di Magliano MP, Cukierman E, Fridman R, Crawford HC2020The American Journal of PathologyOncology, ImmunologyCytonuclear, Multiplex IHC, Highplex FL
CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta, alpha, beta, and gammaGeng J, Chen L, Yuan Y, Wang K, Wang Y, Qin C, Wu G, Chen R, Zhang Z, Wei D, Du P, Zhang J, Lin P, Zhang K, Deng Y, Xu K, Liu J, Sun X, Guo T, Yang X, Wu J, Jiang J, Li L, Zhang K, Wang Z, Zhang J, Yan Q, Zhu H, Zheng Z, Miao J, Fu X, Yang F, Chen X, Tang H, Zhang Y, Shi Y, Zhu Y, Pei Z, Huo F, Liang X, Wang Y, Wang Q, Xie W, Li Y, Shi M, Bian H, Zhu P, Chen Z2021Signal Transduction and Targeted TherapyInfectious DiseaseCytonuclear, Multiplex IHC, Highplex FL
Cross-linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFN?/CXCL10-dependent peripheral T cell recruitment in humanized murine modelCremasco F, Menietti E, Speziale D, Sam J, Sammicheli S, Richard M, Varol A, Klein C, Umana P, Bacac M, Colombetti S, Perro M2021PLOS ONEOncologyCytonuclear, Multiplex IHC, Highplex FL
Interplay of somatic alterations and immune infiltration modulates response to PD-1 blockade in advanced clear cell renal cell carcinomaBraun DA, Hou Y, Bakouny Z, Ficial M, Sant' Angelo M, Forman J, Ross-Macdonald P, Berger AC, Jegede OA, Elagina L, Steinharter J, Sun M, Wind=Rotolo M, Pignon J-C, Cherniack AD, Lichtenstein L, Neuberg D, Catalano P, Freeman GJ, Sharpe AH, McDermott DF, Van Allen EM, Signoretti S, Wu CJ, Shukla SA, Choueiri TK2020Nature MedicineImmuno-oncologyHighplex FL, Partitions (tiles, margins)
IFN-III is selectively produced by cDC1 and predicts good clinical outcome in breast cancerHubert M, Gobbini E, Couillault C, Manh T-PV, Doffin A-C, Berthet J, Rodriguez C, Ollion V, Kielbassa J, Sajous C, Treilleux I, Tredan O, Dubois B, Dalod M, Bendriss-Vermare N, Caux C, Valladeau-Guilemond J2020ImmunologyOncologyHighplex FL, Partitions (tiles, margins)
Androgen Receptor Signalling in Macrophages Promotes TREM-1-mediated Cancer Cell Line Migration and InvasionCioni B, Zaalberg A, van Beihnum J, Melis M, van Brugsteden J, Muraro M, Hooijberg E, Peters D, Hofland I, Lubeck Y, de Jong J, Sanders J, Vivie J, van der Poel J, Griffioen A, Zwart W, Bergman A2021Nature CommunicationsOncologyHighplex FL, Partitions (tiles, margins)
mRNA translation is a therapeutic vulnerability necessary for bladder epithelial transformationJana S, Deo R, Hough R, et al.2021JCI InsightOncologyMultiplex IHC, Highplex FL
Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint BlockadeSrivastava S, Furlan SN, Jaeger-Ruckstuhl CA, Sarvothama M, Berger C, Smythe KS, Garrison SM, Specht JM, Lee SM, Amezquita RA, Voillet V, Muhunthan V, Yechan-Gunja S, Pillai SPS, Rader C, Houghton AM, Pierce RH, Gottardo R, Maloney DG, Riddell SR2020Cancer CellImmuno-oncologyMultiplex IHC, Highplex FL
STAT3 antisense oligonucleotide remodels the suppressive tumor microenvironment to enhance immune activation in combination with anti-PD-L1Proia T, Singh M, Woessner R, Carnevalli LS, Bommakanti G, Magiera L, Srinivasan SS, Grosskurth SE, Collins M, Womack C, Griffin M, Ye M, Cantin S, Russell DL, Xie M, Hughes AM, Deng N, Mele DA, Fawell SE, Barry ST, Reimer C, Barrett JC, McCoon P2020Clinical Cancer ResearchImmuno-oncologyMultiplex IHC, Highplex FL
Neonatal hydrocephalus leads to white matter neuroinflammation and injury in the corpus callosum of Ccdc39 hydrocephalic miceGoulding DS, Vogel RC, Pandya CD, Shula C, Gensel JC, Mangano FT, Goto J, Miller BA2020Journal of NeurosurgeryImmunology, NeuroscienceMultiplex IHC, Highplex FL
Spatially organized multicellular immune hubs in human colorectal cancerPelka K, Hofree M, Chen J, Sarkizova S, Pirl J, Jorgji V, Bejnood A, Dionne D, Ge W, Xu K, Chao S, Zollinger D, Lieb D, Reeves J, Fuhrman C, Hoang M, Delorey T, Nguyen L, Waldman J, Klapholz M, Wakiro I, Cohen O, Albers, J, Smillie C, Cuoco M, Wu J, Su M, Yeung J, Vijaykumar B, Magnuson A, Asinovski N, Moll T, Goder-Reise M, Applebaum A, Brais L, DelloStritto L, Denning S, Phillips S, Hill E, Meehan J, Frederick D, Sharova T, Kanodia A, Todres E, Jane-Valbuena J, Biton M, Izar B, Lambden C, Clancy T, Bleday R, Melnitchouk N, Irani J, Kunitake H, Berger D, Srivastava A, Hornick J, Ogino S, Rotem A, Vigneau S, Johnson B, Corcoran R, Sharpe A, Kuchroo V, Ng K, Giannakis M, Nieman L, Boland G, Arguirre A, Anderson A, Rosenblatt-Rosen O, Regev A, Hachohen N2021CellOncologySpatial Analysis, Highplex FL
Dysregulation of Innate Lymphoid Cells in Patients with Active Rheumatoid Arthritis and Mice with Collagen-Induced ArthritisYang F, Luo X, Zhu W, Li J, Zheng Z, Zhu P2021Mediators of InflammationSpatial Analysis, Highplex FL
Chemokine-Capturing Wound Contact Layer Rescues Dermal HealingSchirmer L, Atallah P, Freudenberg U, Werner C2021Advanced ScienceDermatologyHighplex FL, Layer Thickness
T-regulatory cells predict clinical outcome in soft tissue sarcoma patients: a clinico-pathological studySmolle M, Herbsthofer L, Granegger B, Goda M, Brcic I, Bergovec M, Scheipl S, Prietl B, Pichler M, Gerger A, Rossmann C, Riedl J, Tomberger M, López-García P, El-Heliebi A, Leithner A, Liegl-Atzwanger B, Szkandera J2021British Journal of CancerOncologyHighplex FL, TMA
Multiplex Immunohistochemical Phenotyping of T Cells in Primary Prostate CancerOzbek B, Ertunc O, Erickson A, Vidal I, Alexandre C, Guner G, Hicks J, Jones T, Taube J, Sfanos K, Yegnasubramanian S, DeMarzo A2021medRxivImmuno-oncologyClassifier, Cytonuclear, Highplex FL, Serial Section, Registration
PIK3CA mutation and CNV status and post-chemoradiotherapy survival in patients with cervical cancerMartell K, McIntyre JB, Kornaga EN, Chan AMY, Phan T, Kobel M, Enwere EK, Dean ML, Ghatage P, Lees-Miller SP, Doll CM2020Gynecologic OncologyOncologyClassifier, Highplex FL, TMA
Tissue-resident FOLR2+ macrophages associate with tumor-infiltrating CD8+ T cells and with increased survival of breast cancer patientsRamos, R, Missolo-Koussou Y, Gerber-Ferder Y, Bromley C, Bugatti M, Nunez N, Tosello J, Richer W, Denizeau J, Sedlik C, Caudana P, Kotsias F, Niborski L, Viel S, Bohec M, Lameiras S, Baulande S, Lesage L, Nicolas A, Mesure D, Vincent-Salomon A, Reyal F, Dutertre C, Ginhoux F, Vimeux L, Donnadieu E, Buttard B, Galon J, Zelenay S, Vermi W, Guermonprez P, Piaggio E, Helft J2021bioRxivImmuno-oncologyCytonuclear, Highplex FL, TMA
Oncogenic BRAF, unrestrained by TGF?-receptor signalling, drives right-sided colonic tumorigenesisLeach J, Vlahov N, Tsantoulis P, et al2021Nature CommunicationsOncologyISH/FISH, Highplex FL
Anti-CD47 antibody administration via cisterna magna in proper dosage can reduce perihematomal cell death following intracerebral hemorrhage in ratsSong Y, Wang H, Li F, Huang Q, Zhang Z2021Brain Research BulletinOtherHighplex FL
Local radiotherapy and E7 RNA-LPX vaccination show enhanced therapeutic efficacy in preclinical models of HPV16+ cancerSalomon N, Selmi A, Grunwitz C, Kong A, Stanganello E, Neumaier J, Petschenka J, Diken M, Kreiter S, Tureci O, Sahin U, Vascotto F2021Cancer Immunology, ImmunotherapyOncology, Infectious DiseaseHighplex FL
ACE2-IgG1 fusions with improved in vitro and in vivo activity against SARS-CoV-2Iwanaga N, Cooper L, Rong L, Maness N, Beddingfield B, Qin Z, Crabtree J, Tripp R, Yang H, Blair R, Jangra S, Garcia-Sastre A, Schotsaert M, Chandra S, Robinson J, Srivastava A, Rabito F, Qin X, Kolls J2022iScienceInfectious DiseaseHighplex FL
Tumor MHC Class I Expression Associates with Intralesional Interleukin-2 Response in MelanomaPourmaleki M, Jones C, Ariyan C, Zeng Z, Pirun M, Navarrete D, Li Y, Zhang M, Nandakumar S, Campos C, Nadeem S, Klimstra D, Temple-Oberle C, Brenn T, Kipson E, Schenk K, Stein J, Taube J, White M, Traweek R, Wargo J, Kirkwood J, Gasmi B, Goff S, Corwin A, McDonough E, Ginty F, Callahan M, Schietinger A, Socci N, Mellinghoff I, Hollmann T2022Cancer Research ImmunologyImmuno-oncologyHighplex FL
T cell-mediated anti-tumor immunity cooperatively induced by TGF?R1 antagonism and gemcitabine counteracts reformation of the stromal barrier in pancreatic cancerLi D, Schaub N, Guerin T, Bapiro T, Richards F, Chen V, Talsania K, Kumar P, Gilbert D, Scholmer J, Kim S, Sorber R, Teper Y, Bautista W, Palena C, Ock C, Jodrell D, Pate N, Mehta M, Zhao Y, Kozlov S, Rudloff U2021Molecular Cancer TherapeuticsImmuno-oncologyHighplex FL
Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiomeMcDew-White M, Lee E, Alvarez X, Sestak K, Ling B, Byrareddy S, Okeoma C, Mohan M2022EBioMedicineInfectious DiseaseArea Quantification, Cytonuclear, Highplex FL
Tumor cell-derived exosomes deliver TIE2 protein to macrophages to promote angiogenesis in cervical cancerDu S, Qian J, Tan S, Li W, Liu P, Zhao J, Zeng Y, Xu L, Wang Z, Cai J2022Cancer LettersOncologyArea Quantification, Cytonuclear, Spatial Analysis, Highplex FL
spatialTIME and iTIME: R package and Shiny application for visualization and analysis of immunofluorescence dataCreed J, Wilson C, Soupir A, Colin-Leitzinger C, Kimmel G, Ospina O, Chakiryan N, Markowitz J, Peres L, Coghill A, Fridley B2021BioinformaticsOtherClassifier, Cytonuclear, Highplex FL
Understanding the impact of chemotherapy on the immune landscape of high-grade serous ovarian cancerVanguri R, Benhamida J, Young J, Li Y, Zivanovic O, Chi D, Snyder A, Hollmann T, Mager K2022Gynecologic Oncology ReportsOncologyClassifier, Cytonuclear, Highplex FL
IgA-dominated humoral immune responses govern patients' outcome in endometrial cancerMandal G, Biswas S, Anadon C, Yu X, Gatenbee C, Prabhakaran S, Payne K, Chaurio R, Martin A, Innamarato P, Moran C, Powers J, Harro C, Mine J, Sprenger K, Rigolizzo K, Wang X, Curiel T, Rodriguez P, Anderson A, Saglam O, Conejo-Garcia J2021Cancer ResearchImmuno-oncologyClassifier, Cytonuclear, Highplex FL
Neoadjuvant immunotherapy with nivolumab and ipilimumab induces major pathological responses in patients with head and neck squamous cell carcinomaVos J, Elbers J, Krijgsman O, Traets J, Qiao X, van der Leun A, Lubeck Y, Seignette I, Smit L, Willems S, van den Brekel M, Dirven R, Karakullukcu B, Karssemakers L, Klop W, Lohuis P, Schreuder W, Smeele L, van der Velden L, Tan I, Onderwater S, Jasperse B, Vogel W, Al-Mamgani A, Keijser A, van der Noort V, Broeks A, Hooijberg E, Peeper D, Schumacher T, Blank C, de Boer J, Haanen J, Zuur C2021Nature CommunicationsOncologyClassifier, Highplex FL
Pancreas resident macrophage-induced fibrosis has divergent roles in pancreas inflammatory injury and PDACBaer J, Zuo C, Kang L, Alarcon de la Lastra A, Bocherding N, Knolhoff B, Bogner S, Zhu Y, Lewis M, Zhang N, Kim K, Fields R, Mills J, Ding L, Randolph G, DeNardo D2022bioRxivFibrosisClassifier, Area Quantification, Cytonuclear, Multiplex IHC, Highplex FL, Figure Maker
Progranulin mediates immune evasion of pancreatic ductal adenocarcinoma through regulation of MHCI expressionCheung P, Yang J, Fang R, Borgers A, Krengel K, Stoffel A, Althoff K, Yip C, Siu E, Ng L, Lang K, Cham L, Engel D, Soun C, Cima I, Scheffler B, Striefler J, Sinn M, Bahra M, Pelzer U, Oettle H, Markus P, Smeets E, Aarntzen E, Savvatakis K, Liffers S, Lueong S, Neander C, Bazarna A, Zhang X, Paschen A, Crawford H, Chan A, Cheung S, Siveke J2022Nature CommunicationsOncologyCytonuclear, Spatial Analysis, Highplex FL
The Tumor Immune Landscape and Architecture of Tertiary Lymphoid Structures in Urothelial Cancervan Dijk N, Gil-Himenez A, Silina K, van Montfoort M, Einerhand S, Jonkman L, Voskuilen C, Peters D, Sanders J, Lubeck Y, Broeks A, Hooijberg E, Vis D, van den Broek M, Wessels L, van Rhijn B, van der Heijden M2021Frontiers in ImmunologyImmuno-oncologyCytonuclear, Spatial Analysis, Highplex FL
New Fully Automated Preparation of High Apparent Molar Activity 68Ga-FAPI-46 on a Trasis AiO PlatformDa Pieve C, Braga M, Turton D, Valla F, Cakmak P, Plate K, Kramer-Marek G2022MoleculesOtherCytonuclear, Highplex FL
Retrospective analysis of the efficacy of adjuvant cytokine-induced killer cell immunotherapy combined with chemotherapy in colorectal cancer patients after surgeryLi X, Zhou H, Huang W, Wang X, Meng M, Hou Z, Liao L, Tang W, Xie Y, Wang R, Yu H, Wang L, Zhu H, Wang W, Tan J, Li R2022Clinical & Translational ImmunologyOncologyCytonuclear, Highplex FL
Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 ExpressionCarossino M, Kenney D, O'Connell A, Montarnaro P, Tseng A, Gertje H, Grosz K, Ericsson M, Huber B, Kurnick S, Subramaniam S, Kirkland T, Walker J, Francis K, Klose A, Paragas N, Bosmann M, Saeed M, Balasuriya U, Douam F, Crossland N2022VirusesInfectious DiseaseClassifier, Area Quantification, Highplex FL
Multi-modal molecular imaging maps the correlation between tumor microenvironments and nanomedicine distributionStrittmatter N, Moss J, Race A, Sutton D, Canales J, Ling S, Wong E, Wilson J, Smith A, Howes C, Bunch J, Barry S, Goodwin R, Ashford M2022TheranosticsImmuno-oncologyHighplex FL
An anti-PD-1–GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapyChan S, Belmar N, Ho S, Rogers B, Stickler M, Graham M, Lee E, Tran N, Zhang D, Gupta P, Sho M, MacDonough T, Woolley A, Kim H, Zhang H, Liu W, Zheng P, Dezso Z, Halliwill K, Ceccarelli M, Rhodes S, Thakur A, Forsyth C, Xiong M, Tan S, Iyer R, Lake M, Digiammarino E, Zhou L, Bigelow L, Longenecker K, Judge R, Liu C, Trumble M, Remis J, Fox M, Cairns B, Akamatsu Y, Hollenbaugh D, Farding F, Alvarez H2022Nature CancerImmuno-oncologyArea Quantification, Cytonuclear, Highplex FL
ZEB1 transcription factor promotes immune escape in melanomaPlaschka M, Benboubker V, Grimont M, Berthet J, Tonon L, Lopez J, Le-Bouar M, Balme B, Tondeau G, de la Fouchardiere A, Larue L, Puisieux A, Grinberg-Bleyer Y, Bendriss-Vermare N, Dubois B, Caux C, Dalle S, Caramel J2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Multiplex IHC, Highplex FL
Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretionVeprik A, Denwood G, Liu D, Bakar R, Morfin V, McHugh K, Tebeka N, Vetterli L, Yonova-Doing E, Gribble F, Reimann F, Hoehn K, Hemsley P, Ahnfelt-Ronne J, Rorsman P, Zhang Q, Wet H, Cantley J2022Communications BiologyMetabolismCytonuclear, Highplex FL
Construction of immune related prognostic model for pancreatic ductal adenocarcinoma based on single cell RNA-seq analysisChen K, Wang Q, Liu X, Wang F, Ma Y, Zhang S, Shao Z, Yang Y, Tian X2022Research SquareOncologyCytonuclear, Highplex FL
Extracellular matrix proteins regulate NK cell function in peripheral tissuesBunting M, Vyas M, Requesens M, Langenbucher A, Schiferle E, Manguso R, Lawrence M, Demehri S2022Science AdvancesDermatologyCytonuclear, Highplex FL
Multiplex Immunohistochemical Phenotyping of T Cells in Primary Prostate CancerOzbek B, Ertunc O, Erickson A, Vidal I, Gomes-Alexandre C, Guner G, Hicks J, Jones T, Taube J, Sfanos K, Yegnasubramanian S, DeMarzo A2022The ProstateImmuno-oncologyClassifier, Cytonuclear, Highplex FL, Serial Section, Registration, Figure Maker, TMA
Genomic and single-cell landscape reveals novel drivers and therapeutic vulnerabilities of transformed cutaneous T-cell lymphomaSong X, Chang S, Seminario-Vidal L, de Mingo Pulido A, Tordesillas L, Song X, Reed R, Harkins A, Whiddon S, Nguyen J, Segura C, Zhang C, Yoder S, Sayegh Z, Zhao Y, Messina J, Harro C, Zhang X, Conejo-Garcia J, Berglund A, Sokol L, Zhang J, Rodriguez P, Mule J, Futreal A, Tsai K, Chen P2022Cancer DiscoveryOncologyCytonuclear, Highplex FL, TMA
Tumor immune cell clustering and its association with survival in African American women with ovarian cancerWilson C, Soupir A, Thapa R, Creed J, Nguyen J, Segura C, Gerke T, Schildkraut J, Peres L, Fridley B2022PLOS Computational BiologyCytonuclear, Highplex FL
Immunologic Gene Signature Analysis Correlates Myeloid Cells and M2 Macrophages with Time to Trabectedin Failure in Sarcoma PatientsSchroder B, Zhang Y, Smythe K, Desai P, Thomas A, Viveiros P, Alexiev B, Obeidin F, Chen E, Cranmer L, Wagner M, Jones R, Campbell J, Pierce R, He Q, Pollack S2022CancersOncologyCytonuclear, Highplex FL
Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infectionKenney D, O'Connell A, Turcinovic J, Montanaro P, Hekman R, Tamura T, Berneshawi A, Cafiero T, Abdullatif S, Blum B, Goldstein S, Heller B, Gertje H, Bullitt E, Trachtenberg A, Chavez E, Nono E, Morrison C, Tseng A, Sheikh A, Kurnick S, Grosz K, Bosmann M, Ericsson M, Huber B, Saeed M, Balazs A, Francis K, Klose A, Paragas N, Campbell J, Connor J, Emili A, Crossland N, Ploss A, Douam F2022Cell ReportsInfectious DiseaseArea Quantification, Highplex FL
Improved cognitive impairments by silencing DMP1 via enhancing the proliferation of neural progenitor cell in Alzheimer-like miceZhao H, Wei J, Du Y, Chen P, Liu X, Liu H2022Aging CellNeuroscienceCytonuclear, Highplex FL
Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancerLiu H, Wu J, Xu X, Wang H, Zhang C, Yin S, He Y2022International ImmunopharmacologyOncologyCytonuclear, Spatial Analysis, Highplex FL
Subpopulations of cancer-associated fibroblasts link the prognosis and metabolic features of pancreatic ductal adenocarcinomaHu B, Wu C, Mao H, Gu H, Dong H, Yan J, Qi Z, Yuan L, Dong Q, Long J2022Annals of Translational MedicineOncologyCytonuclear, Highplex FL
SPOP promotes cervical cancer progress by inducing PD-1 move away from PD-L1 in spatial localizationWu J, Wu Y, Guo Q, Chen S, Wang S, Zhu J, Wu X, Ju X2022Research SquareOncologyCytonuclear, Spatial Analysis, Highplex FL, TMA
Spatially mapping the immune landscape of melanoma using imaging mass cytometryMoldoveanu D, Ramsay L, Lajoie M, Anderson-Trocme L, Lingrand M, Berry D, Perus L, Wei Y, Moraes C, Alkallas R, Rajkumar S, Zuo D, Dankner M, Xu E, Bertos N, Najafabadi H, Gravel S, Costantino S, Richer M, Lund A, Del Rincon S, Spatz A, Miller W, Jamal R, Lapointe R, Mes-Masson A, Turcotte S, Petrecca K, Dumitra S, Meguerditchian A, Richardson K, Tremblay F, Wang B, Chergui M, Guiot M, Watters K, Stagg J, Quail D, Mihalcioiu C, Meterissian S, Watson I2022Science ImmunologyOncologyHighplex FL
Pilot Trial of Arginine Deprivation Plus Nivolumab and Ipilimumab in Patients with Metastatic Uveal MelanomaKraehenbuehl L, Holland A, Armstrong E, O'Shea S, Mangarin L, Chekalil S, Johnston A, Bomalaski J, Erinjeri J, Barker C, Francis J, Wolchok J, Merghoub T, Shoushtari A2022CancersOncologyCytonuclear, Highplex FL
Nitric oxide favours tumour-promoting inflammation through mitochondria-dependent and -independent actions on macrophagesDrehmer D, Luiz J, Hernandez C, Alves-Filho J, Hussell T, Townsend P, Moncada S2022Redox BiologyOncologyHighplex FL
Myeloid cell-driven nonregenerative pulmonary scarring is conserved in multiple nonhuman primate species regardless of SARS-CoV-2 infection modalityFears A, Beddingfield B, Chirichella N, Slisarenko N, Kileen S, Redmann R, Goff K, Spencer S, Picou B, Golden N, Bush D, Branco L, Boisen M, Gao H, Montefiori D, Blair R, Doyle-Meyers L, Russel-Lodrigue K, Maness N, Roy C2021bioRxivArea Quantification, Highplex FL
CD4+ T helper 2 cells suppress breast cancer by inducing terminal differentiationBoieri M, Malishkevich A, Guennoun R, Marchese E, Kroon S, Trerice K, Awad M, Park J, Iyer S, Kreuzer J, Haas W, Rivera M, Demehri S2022Journal of Experimental MedicineImmuno-oncolgyCytonuclear, Highplex FL
Higher proportions of CD39+ tumorresident cytotoxic T cells predict recurrence-free survival in patients with stage III melanoma treated with adjuvant immunotherapyAttrill G, Owen C, Ahmed T, Vergara I, Colebatch A, Conway J, Nahar K, Thompson J, da Silva I, Carlino M, Menzies A, Lo S, Palendira U, Scolyer R, Long G, Wilmott J2022Journal of Immuno Therapy of CancerImmuno-oncologyCytonuclear, Spatial Analysis, Highplex FL
Baseline and post-treatment biomarkers of resistance to anti-PD-1 therapy in acral and mucosal melanoma: an observational studyShui I, Liu X, Zhao Q, Kim S, Sun Y, Yearley J, Choudhury T, Webber A, Krepler C, Cristescu R, Lee J2022Journal for ImmunoTherapy of CancerImmuno-oncologyCytonuclear, Highplex FL
Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate speciesFears A, Beddingfield B, Chirichella N, Slisarenko N, Kileen S, Redmann R, Goff K, Spencer S, Picou B, Golden N, Midkiff C, Bush D, Branco L, Boisen M, Gao H, Montefiori D, Blair R, Doyle-Meyers L, Russel-Lodrigue K, Maness N, Roy C2022PLOS PathogensInfectious DiseaseHighplex FL
gp96 Expression in Gliomas and Its Association with Tumor Malignancy and T Cell Infiltrating LevelLi C, Wang Y, Long L, Zhang P, Zhang Y, Ji N2022Hindawi Journal of OncologyImmuno-oncologySpatial Analysis, Highplex FL, Figure Maker
Multiplexed immunofluorescence analysis of CAFmarkers, EZH2 and FOXM1 in Gastric Tissue: Associations with Clinicopathological Parameters and Clinical OutcomesSun H, Wang X, Wang X, Tan C, Weng W, Zhang M, Ni S, Wang L, Huang D, Sheng W, Xu M2022Research SquareOncologyCytonuclear, Highplex FL
Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrixKay E, Paterson K, Riero-Domingo C, Sumpton D, Dabritz J, Tardito S, Boldrini C, Hernandez-Fernaud J, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson L, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson R, Park M, Kirschner K, Miller C, Kamphorst J, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S2022Nature MetabolismOncology, MetabolismCytonuclear, Multiplex IHC, Highplex FL
Integrated single-cell analyses decode the developmental landscape of the human fetal spineYu H, Tang D, Wu H, Li C, Lu Y, He F, Zhang X, Yang Y, Shi W, Hu W, Zeng Z, Dai W, Ou M, Dai Y2022iScienceOtherHighplex FL
Establishing a multiplex imaging panel to study T cell development in the thymus in mouseAllam A, Russell S2022STAR ProtocolsImmunologyHighplex FL
Developing T cells form an immunological synapse for passage through the β-selection checkpointAllam A, Charnley M, Pham K, Russel S2022Journal of Cell BiologyImmunologyHighplex FL
Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor modelsKaeppler J, Chen J, Buono M, Vermeer J, Kannan P, Cheng W, Voukantsis D, Thompson J, Hill M, Allen D, Gomes A, Kersemans V, Kinchesh P, Smart S, Buffa F, Nerlov C, Muschel R, Markelc B2022EMBO ReportsOncologySpatial Analysis, Highplex FL
Non-canonical EphA2 activation underpins PTEN-mediated metastatic migration and poor clinical outcome in prostate cancerSachdeva A, Hart C, Kim K, Tawadros T, Oliveira P, Shanks J, Brown M, Clarke N2022British Journal of CancerOncologyHighplex FL
Approaches to spatially resolving the tumour immune microenvironment of hepatocellular carcinomaMarsh-Wakefield F, Ferguson A, Liu K, Santhakumar C, McCaughan G, Palendira U2022Therapeutic Advances in Medical OncologyReviewMultiplex IHC, Spatial Analysis, Highplex FL

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