Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.

Highplex FL

HIGHPLEX FL

 

Product Overview

Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane.  With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya Vectra® 3 and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Akoya (CODEX® Platform), Ultivue (UltiMapper and InSituPlex® Technology), Fluidigm (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).

In the example shown here, the fluorescent image displays five colors – a membrane bound tumor marker in red, a cytoplasmic marker in cyan, two distinct nuclear markers in green and fuschia and blue DAPI counterstain.  In the markup image, the nucleus, cytoplasm and membranes are colored based on the cell positivity for each marker. Cells negative for all markers are black, cells positive for multiple markers are white, and cells positive for individual makers are colored according to that marker color. When used in conjunction with our Spatial Analysis module, the spatial relationships of these cell populations may be further interrogated.

The Highplex FL module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats. If you don’t see your format listed here, it doesn’t necessarily mean that we don’t support it. Drop an email to info@indicalab.com to check with us directly.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

HIGHPLEX FL

Indica Labs’ Highplex FL module allows for simultaneous analysis of an unlimited number of fluorescent markers in any cellular compartment – nucleus, cytoplasm, and/or membrane.  With the option to define specific cell phenotypes according to marker positivity, this module is ideally suited for applications in immuno-oncology where multiple markers are required to characterize distinct immune and tumor cell populations within the tissue.  It can be used in conjunction with most fluorescent whole-slide image formats, multispectral floating-point tiff images and whole-slide qptiff images from the Akoya Vectra® 3 and Polaris™ platforms, as well as imagery obtained using novel highplexing and ultra-highplexing technologies from Akoya (CODEX® Platform), Ultivue (UltiMapper and InSituPlex® Technology), Fluidigm (Hyperion™ Imaging System) and IONpath (MIBI™ Platform).

In the example shown here, the fluorescent image displays five colors – a membrane bound tumor marker in red, a cytoplasmic marker in cyan, two distinct nuclear markers in green and fuschia and blue DAPI counterstain.  In the markup image, the nucleus, cytoplasm and membranes are colored based on the cell positivity for each marker. Cells negative for all markers are black, cells positive for multiple markers are white, and cells positive for individual makers are colored according to that marker color. When used in conjunction with our Spatial Analysis module, the spatial relationships of these cell populations may be further interrogated.

HALO is compatible with a broad spectrum of image and digital slide formats.   If you don’t see your format listed here, it doesn’t necessarily mean that we don’t support it.  Drop an email to info@indicalab.com to check with us directly.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Here are a few publications that cite use of the Highplex FL module.  Your publication not on the list?  Drop us an email to let us know about it!

TitleAuthorsYearJournalApplicationHALO Modules
Integrative molecular characterization of sarcomatoid and rhabdoid renal cell carcinomaBakouny Z, Braun DA, Shukla SA, Pan W, Gao X, Hou Y, Flaifel A, Tang S, Bosma-Moody A, He MX, Vokes N, Nyman J, Xie W, Nassar AH, Alaiwi SA, Flippot R, Bouchard G, Steinharter JA, Nuzzo PV, Ficial M, Sant’Angelo M, Forman J, Berchuck JE, Dudani S, Bi K, Park J, Camp S, Sticco-Ivins M, Hirsch L, Baca SC, Wind-Rotolo M, Ross-Macdonald P, Sun M, Mary Lee G-SM, Chang SL, Wei XX, McGregor BA, Harshman LC, Genovese G, Ellis L, Pomerantz M, Hirsch MS, Freedman ML, Atkins MB, Wu CJ, Ho TH, Linehan WM, McDermott DF, Heng DYC, Viswanathan SR, Signoretti S, Van Allen EM, Choueiri TK2021Nature CommunicationsOncologyHighplex FL
Human ileal organoid model recapitulates clinical incidence of diarrhea associated with small molecule drugsBelair DG, Visconti RJ, Hong M, Marella M, Peters MF, Scott CW, Kolaja KL2020Toxicology in VitroToxicologyHighplex FL
IgA transcytosis and antigen recognition govern ovarian cancer immunityBiswas S, Mandal G, Payne KK, Anadon CM, Gatenbee CD, Chaurio RA, Costich TL, Moran C, Harro CM, Rigolizzo KE, Mine JA, Trillo-Tinoco J, Sasamoto N, Terry KL, Marchion D, Buras A, Wenham RM, Yu X, Townsend MK, Tworoger SS, Rodriguez CR, Anderson AR, Conejo-Garcia JR2021NatureImmuno-oncologyHighplex FL
Spatial Clustering of CD68+ Tumor Associated Macrophages with Tumor Cells is Associated with Worse Overall Survival in Metastatic Clear Cell Renal Cell CarcinomaChakiryan NH, Kimmel GJ, Kim Y, Hajiran A, Aydin AM, Zemp L, Katende E, Nguyen J, Lopez-Blanco N, Chahoud J, Spiess PE, Fournier M, Dhillon J, Wang L, Moran-Segura C, El-Kenawi A, Mulé J, Altrock PM, Manley BJ2021bioRxivImmuno-oncologyHighplex FL
Multiomic analysis and immunoprofiling reveal distinct subtypes of human angiosarcomaChan JY, Lim JQ, Yeong J, Ravi V, Guan P, Boot A, Tay TKY, Selvarajan S, Nasir NDM, Loh JH, Ong CK, Huang D, Tan J, Li Z, Ng CC-Y, Tan TT, Masuzawa M, Sung KW-K, Farid M, Quek RHH, Tan NC, Teo MCC, Rozen SG, Tan P, Fotreal A, The BT, Soo KC2020The Journal of Clinical InvestigationImmuno-oncologyHighplex FL
Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma Cloughesy TF, Mochizuki AY, Orpilla JR, Hugo W, Lee AH, Davidson TB, Wang AC, Ellingson BM, Rytlewski JA, Sanders CM, Kawaguchi ES, Du L, Li G, Yong WH, Gaffey SC, Cohen AL, Mellinghoff IK, Lee EQ, Reardon DA, O’Brien BJ, Butowski NA, Nghiemphu PL, Clarke JL, Arrillaga-Romany IC, Colman H, Kaley TJ, de Groot JF, Liau LM, Wen PY, Prins RM2019Nature MedicineOncology, Immuno-oncologySpatial Analysis, Highplex FL
Characterization of Macrophages and Osteoclasts in the Osteosarcoma Tumor Microenvironment at Diagnosis: New Perspective for Osteosarcoma Treatment?Gomez-Brouchet A, Gilhodes J, Van Acker N, Brion R, Bouvier C, Assemat P, Gaspar N, Aubert S, Guinebretiere J-M, Marie B, Larousserie F, Entz-Werlé N, de Pinieux G, Mascard E, Gouin F, Brousset P, Tabone M-D, Jimenez M, Le Deley M-C, Blay J-Y,
Brugieres L , Piperno-Neumann S, Rédini F
2021CancersImmuno-oncologyHighplex FL
Tumoral PD-1hiCD8+ 1 T cells are partially exhausted and 2 predict favorable outcome in triple-negative breast cancer Guo L, Cao C, Goswami S, Huang X, Ma L, Guo Y, Yang B, Li T, Chi Y, Zhang X, Wu J2020Clinical ScienceImmuno-oncologyHighplex FL
Reduction of Global H3K27me3 Enhances HER2/ErbB2 Targeted TherapyHirukawa A, Singh S, Wang J, Rennhack JP, Swiatnicki M, Sanguin-Gendreau V, Zuo D, Daldoul K, Lavoie C, Park M, Andrechek ER, Westbrook TF, Harris LN, Varadan V, Smith HW, Muller WJ2019Cell ReportsOncologyHighplex FL
Macrophage-Derived CXCL9 and CXCL10 Are Required
for Antitumor Immune Responses Following Immune
Checkpoint Blockade
House IG, Savas P, Lai J, Chen AXY, Oliver AJ, Teo ZL, Todd KL, Henderson MA, Giuffrida L, Petley EV, Sek K, Mardiana S, Gide TN, Quek C, Scolyer RA, Long GV, Wilmott JS, Loi S, Darcy PK, Beavis PA2019Clinical Cancer ResearchImmuno-oncologyHighplex FL
A single dose of neoadjuvant PD-1 blockade predicts clinical outcomes in resectable melanomaHuang AC, Orlowski RJ, Xu X, Mick R, George SM, Yan PK, Manne S, Kraya AA, Wubbenhorst B, Dorfman L, D’Andrea K, Wenz BM, Liu S, Chilukuri L, Kozlov A, Carberry M, Giles L, Kier MW, Quagliarello F, McGettigan S, Kreider K, Annamalai L, Zhao Q, Mogg R, Xu W, Blumenschein WM, Yearley JH, Linette GP, Amaravadi RK, Schuchter LM, Herati RS, Bengsch B, Nathanson KL, Farwell MD, Karakousis GC, Wherry EJ, Mitchell TC2019Nature MedicineOncology, Immuno-oncologyHighplex FL
CXCR4+ cells are increased in lung tissue of patients with idiopathic pulmonary fibrosisJaffar J, Griffiths K, Oveissi S, Duan M, Foley M, Glaspole I, Symons K, Organ L, Westfall G2020Respiratory ResearchFibrosisHighplex FL
Neutrophil content predicts lymphocyte depletion and anti-PD1 treatment failure in NSCLCKargl J, Zhu X, Zhang H, Yang GHY, Friesen TJ, Shipley M, Maeda DY, Zebala JA, McKay-Fleisch J, Meredith G, Mashadi-Hossein A, Baik C, Pierce RH, Redman MW, Thompson JC, Albelda SM, Bolouri H, Houghton AM2019JCI InsightHighplex FL
CD30+OX40+ Treg is associated with improved overall survival in colorectal cancerLam JH, Hong M, Koo S-L, Chua CWL, Lim KL, Wee F, Wan WK, Leow WQ, Yeo JG, Tan IBH, Yeong J, Lim TKH, Lim TS2021Cancer Immunology, ImmunotherapyImmuno-oncologyHighplex FL
CXCR3 enables recruitment and site-specific bystander activation of memory CD8+ T cellsMaurice NJ, McElrath MJ, Andersen-Nissen E, Frahm N, Prlic M2019Nature CommunicationsImmunologyHighplex FL
MYB-NFIB gene fusions identified in archival adenoid cystic carcinoma tissue employing NanoString analysis: an exploratory study McIntyre JB, Ko JJ, Siever J, Chan AMY, Simpson RHW, Hao D, Lau HY2019Diagnostic PathologyOncologyClassifier, Highplex FL
Sustained Helicobacter pylori infection accelerates gastric dysplasia in a mouse modelO’Brien VP, Koehne AL, Dubrulle J, Rodriguez AE, Leverich CK, Kong VP, Campbell JS, Pierce RH, Goldenring JR, Choi E, Salama NR2021Life Science AllianceImmuno-oncologyHighplex FL
Locally confined IFNɣ production by CD4+ T cells provides niches for murine cytomegalovirus
4 replication in the salivary gland
Oderbolz J, Zangger N, Zimmerman L, Sandu I, Starruß J, Graw F, Oxenius A2021bioRxivClassifier, Spatial Analysis, Highplex FL
Pulmonary Mycobacterium tuberculosis control associates with CXCR3- and CCR6-expressing antigen-specific Th1 and Th17 cell recruitmentShanmugasundaram U, Bucsan AN, Ganatra SR, Ibegbu C, Quezada M, Blair RV, Alvarez X, Velu V, Kaushal D, Rengarajan J2020JCI InsightInfectious DiseaseHighplex FL, HALO AI
T-cell Localization, Activation, and Clonal Expansion in Human Pancreatic Ductal Adenocarcinoma Stromnes IM, Hulbert A, Pierce RH, Greenberg PD, Hingorani SR2017Cancer Immunology ResearchOncology, Immuno-oncologyHighplex FL
Rova-T enhances the anti-tumor activity of anti-PD1 in a murine model of small cell lung cancer with endogenous Dll3 expressionVitorino P, Chuang C-H, Iannello Z, Zhao X, Anderson W, Ferrando R, Zhang Z, Madhavan S, Karsunky H, Saunders LR2020Translational OncologyImmuno-oncologyHighplex FL
Validation and characterisation of prognostically significant PD-L1+ immune cells in HPV+ oropharyngeal squamous cell carcinomaYoung RJ, Bressel M, Porceddu S, Cernelc J, Savas P, Liu H, Urban D, Thai AA, Cooper C, Fua T, Neeson P, Rischin D, Solomon B2019Oral OncologyOncology, Infectious DiseaseClassifier, Highplex FL
Safety, immunogenicity, and clinical efficacy of durvalumab in combination with folate receptor alpha vaccine TPIV200 in patients with advanced ovarian cancer: a phase II trialZamarin D, Walderich S, Holland A, Zhou Q, Iasonos AE, Torrisi JM, Merghoub T, Chesebrough LF, Mcdonnell AS, Gallagher JM, Li Y, Hollmann TJ, Grisham RN, Erskine CL, Block MS, Knutson KL, O'Cearbhaill RE, Aghajanian C, Konner JA2020Journal for ImmunoTherapy of CancerImmuno-oncologyHighplex FL
Trajectory and Functional Analysis of PD-1high CD4+CD8+ T Cells in Hepatocellular Carcinoma by Single-Cell Cytometry and Transcriptome SequencingZheng B, Wang D, Qiu X, Luo G, Wu T, Yang S, Li Z, Zhu Y, Wang S, Wu R, Sui C, Gu Z, Shen S, Jeong S, Wu Z, Gu J, Wang H, Chen L2020Advanced ScienceImmuno-oncologyHighplex FL
Genetic alterations and expression characteristics of ARID1A impact tumor immune contexture and survival in early-onset gastric cancerZou J, Qin W, Wang L, Wang Y, Shen J, Xiong W, Yu S, Song S, Ajani JA, Lin S-Y, Mills GB, Yuan X, Chen J, Peng G2020American Journal of Cancer ResearchImmuno-oncologyMicroglia, Highplex FL
CD38 in Advanced Prostate CancersGuo C, Crespo M, Gurel B, Dolling D, Rekowski J, Sharp A, Petremolo A, Sumanasuriya S, Rodrigues DN, Ferreira A, Pereira R, Figueiredo I, Mehra N, Lambros MBK, Neeb A, Gil V, Seed G, Terstappen L, Alimonti A, Drake CG, Yuan W, de Bono JS2021European UrologyOncologyClassifier, Highplex FL
Influence of tumor-infiltrating immune cells on local control rate, distant metastasis, and survival in patients with soft tissue sarcomaSmolle MA, Herbsthofer L, Goda M, Granegger B, Brcic I, Bergovec M, Scheipl S, Prietl B, El-Heliebi A, Pichler M, Gerger A, Posch F, Tomberger M, López-García P, Feichtinger J, Baumgartner C, Leithner A, Liegl-Atzwanger B, Szkandera J2021OncoImmunologyImmuno-oncologyHighplex FL
Activated Regulatory T-Cells, Dysfunctional and SenescentT-Cells Hinder the Immunity in Pancreatic CancerSivakumar S, Abu-Shah E, Ahern DJ, Arbe-Barnes EH, Jainarayanan AK, Mangal N, Reddy S, Rendek A, Easton A, Kurz E, Silva M, Soonawalla Z, Heij LR, Bashford-Rogers R, Middleton MR, DUstin ML2021cancersClassifier, Multiplex IHC, Spatial Analysis, Highplex FL
The immune suppressive microenvironment affects efficacy of radio-immunotherapy in brain metastasisNiesel K, Schulz M, Anthes J, Alekseeva T, Macas J, Salamero-Boix A, Mockl A, Oberwahrenbrock T, Lolies M, Stein S, Plate KH, Reiss Y, Rodel F, Sevenich L2021EMBO Molecular MedicineImmuno-oncologySpatial Analysis, Highplex FL
Prognostic role of proliferating CD8+ cytotoxic Tcells in human cancersBlessin NC, Li W, Mandelkow T, Jansen HL, Yang C, Raedler JB, Simon R, Büscheck F, Dum D, Luebke AM, Hinsch A, Möller K, Menz A, Bernreuther C, Lebok P, Clauditz T, Sauter G, Marx A, Uhlig R, Wilczak W, Minner S, Krech T, Fraune C, Höflmayer D, Burandt E, Steurer S2021Cellular OncologyImmuno-oncologyHighplex FL, TMA
Engineered red blood cells as an off-the-shelf allogeneic anti-tumor therapeuticZhang X, Lou M, Dastagir SR, Nixon M, Khamhoung A, Schmidt A, Lee A, Subbiah N, McLaughlin DC, Moore CL, Gribble M, Bayhi N, Amin V, Pepi R, Pawar S, Lyford TJ, Soman V, Mellen J, Carpenter CL, Turka LA, Wickham Tj, Chen TF2021Nature CommunicationsOncologyObject Colocalization, Highplex FL

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.