Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.
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Cytonuclear IHC

Cytonuclear IHC

 

Product Overview

The Cytonuclear IHC module measures cell-by-cell IHC positivity for single or multi stain applications. Each cell is measured for cytoplasmic or nuclear positivity of every individual stain as well as the colocalization of different marker combinations. Positive cells are binned and assigned an intensity score (0, 1+, 2+, and 3+) based on stain optical density. The module also reports a variety of other metrics relating to cell/nuclear size and stain intensity. Optionally, the tool can output the complete table of cell data including individual cell sizes and optical density measurements for each stain in each cellular compartment. This module has the option to define specific cell phenotypes according to marker positivity, allowing users to define and count specific cell populations based off of defined positivity and negativity rules.

In the first example shown above, the module is configured to output an intensity markup for a single IHC marker (DAB). Cells below the positivity threshold for the DAB marker are blue and cells above the positivity threshold are scoring according to optical density thresholds set in the module, weak positive (1+) in yellow, moderate positive (2+) in orange and strong positive (3+) in red.

In the second example, the module is used to analyze a dual-stain IHC and a co-expression mark-up is generated. Cells positive for the red cytoplasmic marker only are red, cells positive for the brown cytoplasmic marker are green and dual positive cells are cyan.

The Cytonuclear IHC module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

Cytonuclear IHC

The Cytonuclear IHC module measures cell-by-cell IHC positivity for single or multi stain applications. Each cell is measured for cytoplasmic or nuclear positivity of every individual stain as well as the colocalization of different marker combinations. Positive cells are binned and assigned an intensity score (0, 1+, 2+, and 3+) based on stain optical density. The module also reports a variety of other metrics relating to cell/nuclear size and stain intensity. Optionally, the tool can output the complete table of cell data including individual cell sizes and optical density measurements for each stain in each cellular compartment. This module has the option to define specific cell phenotypes according to marker positivity, allowing users to define and count specific cell populations based off of defined positivity and negativity rules.

In the first example shown above, the module is configured to output an intensity markup for a single IHC marker (DAB). Cells below the positivity threshold for the DAB marker are blue and cells above the positivity threshold are scoring according to optical density thresholds set in the module, weak positive (1+) in yellow, moderate positive (2+) in orange and strong positive (3+) in red.

In the second example, the module is used to analyze a dual-stain IHC and a co-expression mark-up is generated. Cells positive for the red cytoplasmic marker only are red, cells positive for the brown cytoplasmic marker are green and dual positive cells are cyan.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.