Indica
Backed by cutting edge technology, an uncompromising focus on ease-of-use and dedicated customer service, Indica Labs’ software and services are being used to make vital discoveries in pathology labs and research organizations around the world.
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Islet Quantification

ISLET QUANTIFICATION

 

Product Overview

The Islet Quantification module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and/or nuclear positivity for up to two stains.

In the first example, the software is configured to identify islets using two cytoplasmic islet-specific stains, red and brown. Neutral cells are marked blue, red stain positive cells are marked red, brown positive cells are marked green and dual positive cells are marked yellow. The islet area is yellow and the boundaries are demarked with a black lines.

In addition to measuring cytoplasmic positivity, this tool can also be configured to detect positivity within islet nuclei as shown in the second example.  Here, proliferating beta cells are identified as dual positive for insulin (red stain) and Ki67 (brown nuclear stain).   Insulin positive cells are marked red in the cytoplasm and Ki67 positive cells are marked green in the nucleus.

The module reports islet counts, and islet measurements (diameter & area), islet cell counts (beta cells, positive cells, dual positive cells, and negative cells), and a histogram of islet frequency by diameter ranges.

The Islet Quantification module integrates seamlessly into the HALO™ platform which is compatible with a number of third party systems and  file formats.

Contact info@indicalab.com for product demonstration and pricing information or upload some images for a free trial.

ISLET QUANTIFICATION

The Islet Quantification module counts and measures pancreatic islets and classifies cells within each islet. Islet cells can be measured for cytoplasmic and/or nuclear positivity for up to two stains.

In the first example, the software is configured to identify islets using two cytoplasmic islet-specific stains, red and brown. Neutral cells are marked blue, red stain positive cells are marked red, brown positive cells are marked green and dual positive cells are marked yellow. The islet area is yellow and the boundaries are demarked with a black lines.

In addition to measuring cytoplasmic positivity, this tool can also be configured to detect positivity within islet nuclei as shown in the second example.  Here, proliferating beta cells are identified as dual positive for insulin (red stain) and Ki67 (brown nuclear stain).   Insulin positive cells are marked red in the cytoplasm and Ki67 positive cells are marked green in the nucleus.

The module reports islet counts, and islet measurements (diameter & area), islet cell counts (beta cells, positive cells, dual positive cells, and negative cells), and a histogram of islet frequency by diameter ranges.

HALO is compatible with a broad spectrum of image and digital slide formats.  Yours not on the list? Email us your requirements.

Non-proprietary (JPG, TIF)
Nikon (ND2)
3D Histech (MRXS)
Perkin Elmer (QPTIFF, component TIFF)
Olympus (VSI)
Hamamatsu (NDPI, NDPIS)
Aperio (SVS, AFI)
Zeiss (CZI)
Leica (SCN, LIF)
Philips (Requires Philips IMS)

Here are a few publications that cite use of our Islet IHC or FL modules.  Your publication not on the list?  Drop us an email to let us know about it!

Title Author Year Journal
Single-Cell RNAseq Reveals That Pancreatic β-Cells From Very Old Male Mice Have a Young Gene Signature Christopher Daly, Xunbao Duan, and Gavin Thurston 2016 Endocrinology

Use the form below to upload up to three images for us to analyze using HALO.  If the form does not display, please click here.